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Prevention and control measures of pasteurelloidosis in cattle

Author:

Technical Service Department-Zhou Tingxuan, He Qiao


1                       Multocidal pasteurellosis is also known as hemorrhagic septicemia, referred to as bovine failure, pathogenic bacteria multocidal pasteurellosis, cattle are susceptible animals, and the main route of transmission is digestive tract and respiratory tract. The disease will cause huge economic losses to the cattle industry, and vaccination is the main measure to prevent and control the disease.

[1] Pathogen

The pathogen of this disease is Pasteuella mulocidae, a fine cocci bacterium, which is a gram-negative bacterium. Pasteuella mulocidae can be divided into five serotypes A, B, D, E and F according to passive hemagglutination test for capsular antigen (K antigen), of which the following three types have pathogenic effects on cattle:
Type A: can cause bovine pneumonia, severe mastitis;

Type B: Can cause hemorrhagic septicemia in cattle;

  Type E: can cause hemorrhagic septicemia in cattle and buffalo;

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▲ Pasteurellosis multocides

[2] popular characteristics

1. Epidemic characteristics: local or sporadic. According to statistics, 45% of healthy cattle are infected with bacteria. Once the disease occurs, the mortality rate of cattle can reach 80% or even more than 90% [1]. China Agricultural Network reported on July 4, 2020 in the article "Current Epidemic Situation of Cattle Disease and Effective Response" that from 1990 to 2007, 640 sick cattle were defeated and 158 died, with an average mortality rate of 24.7. In 2015, haemorrhagic septicemia broke out in Guiqing Cattle Farm, Zhaotong City, Zhaoyang District, and the incidence rate of cattle reached 80%. Comprehensive prevention and control measures are the key to prevent Pasteurellosis multocida.

2. Source of infection: diseased cattle and other bacteria-carrying animals and resistant animals.

3. Route of transmission: digestive tract, oral or respiratory tract (droplet infection), skin, mucosal wounds, blood-sucking insects, etc.

4. Endogenous infection: Pasteurellosis multocida is a common bacterium carried by cattle. Due to cold, muggy, drastic climate change, humidity, crowding, poor ventilation in pens, lack of nutrition, feed mutation, long-distance transportation, parasitic diseases and other incentives, the resistance of livestock is reduced, and the pathogen can take the opportunity to enter the blood through lymph to cause endogenous infection;

5. Exogenous infection: fodder, drinking utensils and external environment contaminated by diseased cattle are transmitted to healthy cattle through digestive tract; Bacteria discharged by coughing and sneezing are transmitted to healthy cattle through respiratory tract through droplets; Infected by blood-sucking insect media and skin wounds, the above are collectively referred to as exogenous infection.

6. It can be transmitted across species. For example, Pasteurelmefaciens multicides can be transmitted to buffaloes at individual times.

[3] clinical symptoms

The clinical symptoms of the sick cow are persistent high fever, dyspnea, coarse hair, flushing of conjunctiva, loss of appetite, listlessness, mucous fluid secretion at the mouth and nose, abdominal pain, etc. At the beginning of the disease, the stool was porridge, gradually liquid and blood feces. The incubation period of the disease is usually 2 ~ 5d, and the course of the disease is 1~2 d. According to the clinical symptoms, bovine pasteurellesiosis can be divided into the following three symptoms.

  1, defeat blood type

The body temperature of the sick cow increased to 41~42 ℃, constipation first, then diarrhea, stool was pasty or mucous, bloody stool was visible in the later stage, died due to body failure, body temperature dropped before death, natural hole bleeding after death, and the course of disease was relatively short, about 0.5~1 d.

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Post-mortem nasal bleeding in sick cows

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▲ Anal bleeding after death of sick cattle

2. Edema type

High fever, difficulty breathing, poor diet, stop rumination and other symptoms.

The head, throat, neck, chest and other parts of the inflammatory edema, high swelling of the tissues around the tongue, sometimes the tongue out of the mouth, dark red, dysphagia, high dyspnea, skin and mucous membrane cyanosis.

Niu Xi lie down, the conjunctiva becomes red, the course of disease is relatively short, 0.5~1.5 days, if not timely effective treatment measures, the sick cow will eventually suffocate and die.

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▲ Sick cow chest edema

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Head edema of sick cattle

3, pneumonia type

The lungs of sick cattle have wet rales and pleural friction, cough symptoms become serious, later for wet cough, outflow of foam or viscous nasal fluid, body temperature rise, and other symptoms are roughly the same as the symptoms of sepsis. In the late stage of the disease, the breathing mode of the sick cow is mouth breathing, head and neck straightening, mouth stretching tongue panting, and the course of the disease is prolonged, reaching more than a week.

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Sick cow mouth breathing

[4] Diagnosis

In production, it can be diagnosed according to clinical symptoms and typical pathological changes, and can be diagnosed by laboratory diagnosis.

1. Clinical diagnosis

The septicemic type usually has multiple bleeding symptoms; the edematous type is swelling of the head and throat; and the pneumonic type has fibrinous pleuropneumonia symptoms on both sides and anterior lower parts of the lungs.

2. Laboratory diagnosis methods

Using methylene blue staining microscopy, if a large number of small bacilli with light central coloring, deep coloring at both ends and oval shape are found in the smear, the diagnosis can be obtained [2].

[5] Prevention and Control

1. Immunization

Pasteuella multocida is a conditional pathogen with acute onset and rapid death, especially for septic and edematous types. The most effective way to prevent the disease in production is to do a good job in immunization of cattle.

Inoculation method: Inactivated pasteurelloosis vaccine is used for prevention. 6 mL is injected under the skin or intramuscularly for cattle weighing more than 100kg, and 4mL is injected for calves weighing less than 100kg. Enhanced immunization is carried out one month after the initial immunization is completed. Enhanced immunization is carried out every 9 months after the basic immunization is completed. Immunization is carried out every 6 months in high-incidence areas.

2. Strengthen feeding management

A, pasture should have a good breeding environment, focusing on comfort;

B, to ensure the full price of the diet;

C. Prohibition of mixed breeding of livestock and poultry;

D, in the stress response to increase with anti-stress and improve immunity additives added to the diet;

E, when introducing cattle, try to introduce healthy cattle after immunization;

F, do a good job of daily disinfection;

G. Take isolation treatment for cattle that have become ill.

 

 

References:
[1] WU Jin-gang. Prevention and Diagnosis of Pasteurellis Disease in Cattle [J]. Animal Husbandry and Veterinary Medicine, 2016,8(05):188.

[2] Pang Chuanwen. Diagnosis and treatment of pasteurellosis in cattle breeding [J]. China Animal Husbandry and Veterinary Digest, 2017,33(11):174.

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